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KMID : 0903519840270040238
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1984 Volume.27 No. 4 p.238 ~ p.244
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Production of Inulase Using Jerusalem Artichoke Tuber Extract
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김수일/Kim, Su Il
변시명/최원상/최용경/Byun, Si Myung/Choi, Weon Sang/Choe, Yong Kyung
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Abstract
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To produce inulase from Kluyveromyces fragilis No. 351 using Jerusalem artichoke tuber extract, optimization of the condition was conducted. As results, the optimal concentration of artichoke tuber extract was 3.5% and bactocasitone showed better production than yeast extract. The optimal temperature and pH were 30℃ and 5.5, respectively. The addition of NH₄ H₂PO₄ increased the enzyme production. Inulase synthesis was growth-associated and the enzyme production increased as concentration of dissolved oxygen increased. A higher quantity of industrial grade inulase was prepared by the combination of ultrafiltration and ethanol precipitation with 72% recovery.
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